Fatty Acid Methyl Ester (FAME) or biodiesel is a biofuel produced by transesterification of vegetable oils. It has similar properties to fossil Diesel fuel, and is therefore suitable for the operation of Diesel engines. The British Standard specification for Diesel allows a maximum FAME content of up to 7 vol.% (B7).

PropertyUnits  LimitsTest method (notes)
FAME content% (m/m)96.5-EN 14103
Density at 15 °C (b)kg/m3860900EN ISO 3675 (c)
Viscosity at 40 °C (d)mm2/s3.505.00EN ISO 3104
Flash point (e)°C101-EN ISO 2719
Cetane number (e), (h)-51.0-EN ISO 5165
Copper strip corrosion (3 h at 50 °C)Ratingclass 1 EN ISO 2160
Oxidation stability (at 110 °C)h8.0-EN 14112 (c), EN 15751
Acid valuemg KOH/g-0.50EN 14104
Acid valueg iodine/100-0.50EN 14104
Iodine valueg iodine/100-120.0EN 14111 (c)
Linolenic acid methyl ester% (m/m)-12.0EN 14103
Polyunsaturated (•4 double bonds)    
methyl esters% (m/m)-1.00EN 15779
Methanol content% (m/m)-0.20EN 14110
Monoglyceride content% (m/m)-0.70 (j)EN 14105
Diglyceride content% (m/m)-0.20EN 14105
Triglyceride content% (m/m)-0.20EN 14105
Free glycerol% (m/m)-0.02EN 14105 (c)
Water contentmg/kg-500EN ISO 12937
Total contaminationmg/kg-24EN 12662
Sulfated ash content% (m/m)-0.02ISO 3987, EN 14108 (c)
Sulfur contentmg/kg-10.0EN 14109, EN 14538
Group I metals (Na+K) emg/kg-5.0EN 14108 (c), EN 14109
Group II metals (Ca+Mg)mg/kg-5.0EN 14538
Phosphorus contentmg/kg-4.0EN 14107 (c), EN 16294



a. See 5.5.1. in EN 14214:2012+A1:2014.
b. Density may be measured over a range of temperatures from 20 °C to 60 °C. Temperature correction shall be made according to the formula given in Annex B in EN 14214:2012+A1:2014.
c. See 5.5.2. in EN 14214:2012+A1:2014
d. If CFPP is •20 °C or lower, the viscosity shall be measured at •20 °C. The measured value shall not exceed 48 mm2/s. In this case, EN ISO 3104 is applicable without the precision data owing to non-Newtonian behaviour in a two-phase system.
e. See Annex A for precision data in EN 14214:2012+A1:2014.
f. Procedure A shall be applied. Only a flash point test apparatus equipped with a suitable detection device (thermal or ionisation detection) shall be used.
g. A 2 ml sample and apparatus equipped with a thermal detection device shall be used. See also 5.5.2. in EN 14214:2012+A1:2014
h. See 5.5.3. in EN 14214:2012+A1:2014
i. An additional determination method has been developed by CEN, but first needs to be assessed for actual field performance prediction [3].
j. For use as an extender to diesel fuel Table 3 applies in EN 14214:2012+A1:2014.
k. A lower limit may come into force after validation by work on the measurement standard precision.
l. A lower limit of 2,5 mg/kg may come into force after validation work on the measurement standard and on engine oil impacts.


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